Composite

Part:BBa_K3105671

Designed by: Jonas Gockel   Group: iGEM19_Uppsala_Universitet   (2019-10-11)


HRP-2A-eGFP

Part to co-express horseradish peroxidase (HRP) and enhanced green-fluorescent protein (eGFP) in Pichia pastoris. The 2A-peptide will facilitate cleavage of the polypeptide during translation, leading to separate units of HRP and eGFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 880
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 961
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None